Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger NucleasesComparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 GenesCaco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include: A functional knockout of the MRP4 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP4 substrate The vial format enables the MRP4 knockout cells to be included in standard drug transporter protocols Human assay with no interference from animal inhibitors Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP4 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC4 (MRP4) efflux transporter.

Legal Information

ADME/Tox Cell Lines License